Conclusion
Halothiobacillus neapolitanus, a chemolithoautotrophic bacteriium, was successfully isolated and tested for its ability to oxidize thiosulfate. TMN containing 52 mM phosphate buffer pH 7.3, and 10 g/L thiosulfate was the optimum medium for HNT growth and thiosulfate oxidation. The research findings suggest the potential application of this bacterium in the biotrickling filter process of simultaneous hydrogen sulfide and carbon dioxide removal from biogas.