Conserved sequence regions and laccase expression in Pleurotus ostreatus. A, Conserved sequence regions; the numbers
above the sequence indicate conserved histidine residues; B, Arrangement of histidine residues in the catalytically important T1 and T2/T3 centers; coordinate bonds between the amino acid residues and copper ions in the laccase structure were redrawn using the laccase structure of Trametes versicolor (PDB code: 1GYC); C, RNA-Seq analysis of laccase genes in the mycelia, primordia, and fruiting bodies of P. ostreatus. RPKM, Reads Per Kilobase per Million mapped reads.