For the determination of long-chain fatty acids (C14-C18) this
SBSE extract was evaporated and methylated with 0.1 mL of BF3-
methanol 14% during 20 min at 95 C. Reaction was stopped by the
addition of 0.1 mL of water, and after shaking in an ultrasonic bath,
long-chain fatty acidmethyl esters were extracted with 0.1mL of hexane in
an ultrasonic bath for 2 min. GC separation was the same as described
above.