The four prepared substrates were separately packed into polythene bags and tied up for sterilization. Boiling drum containing stacks of sticks and water up to the level of the sticks was used for sterilization. The substrates were packed into the drum and covered with fresh plantain leaves in order to generate enough heat. The substrates were steam-sterilized for three hours and allowed to cool while still in the drum, they were taken to mushroom house and poured separately on sterile polythene sheets on a table. The spawn was sprinkled on the substrates covered with sterile polythene sheet and watered daily to maintain a high relative humidity of between 75 – 80%.