Results and Discussion
Calli of B. rotunda were induced from shoot base explants on
MS medium supplemented with 1 mg L-1 NAA, biotin and
IAA, 2 mg L-1
2,4-D and 30 g L-1
sucrose. The percentage of
explants that produced callus was 53.4%. Swelling of the
explants was observed within one to two weeks, followed by
emergence of callus after another two weeks. Callusing
commenced at the cut surfaces of the explants and finally
covered up the surface of the explants completely. After two
to three times of subcultures, rapid increase in the callus mass
was observed in the medium. The callus developed in this
medium was mixed friable and compact, cream coloured