In the field, Zn deficiency occurs naturally, and the symptoms
of deficiency develop depending on the degree of stress (Brennan
et al., 1993). Visual symptoms of Zn deficiency only occur in cases
of relatively severe deficiency (Alloway, 2008) and are described
as stunted growth, small leaves, chlorosis, rosette and even shoot
die back (Swietlik, 2002; Sharma et al., 2013). Rosette and little leaf
at the shoot tip are the most characteristic symptoms of Zn def