There is increasing interest in producing novel high quality vinegars. In this work, we examined the effect of biologicalageing of white wine on its nitrogen composition (viz. amino acids, urea and ammonium ion, which constitute themain sources of nitrogen for acetic bacteria during the acetification process) with a view to confirming the suitabilityof aged wine for producing quality vinegar.Available nitrogen contents in biologically aged wine were lower than in young (unaged) wine; this resulted in aslightly lower acetification rate and production with the former. The nitrogen composition of the two vinegars wasvery similar, with l-proline and l-cysteine as the major amino acids. By exception, only the vinegar from the youngwine contained ammonium ion. The ageing process by flor yeast produces urea which is eliminated by the bacteriain the next stage.Available nitrogen for use by acetic bacteria in biologically aged wine is seemingly no limiting factor for acetifica-tion. The problem posed by the formation of urea during wine ageing was suppressed by the subsequent acetificationprocess. The study could be an example of how different microorganisms use the available substrate in serialbiotransformations