Molecular analysis of antibiotic resistance gene reveals the presence of 11 genes conferring resistance to different classes of antibiotics. Six different genes conferring resist- ance against tetracycline were mostly detected at frequencies ranging from 11.8% (tet K) to 68.8% (tetM), suggesting that resistance to this class of drug may be genetically mediated as a result of long-term use. Similarly, high detection of blaTEM conferring resistance to β-lactam was observed at a frequency of 56.4% and supports the nd- ing of Momtaz et al. (2012), who reported high rate of detection of resistant genes conferring resistance to tet- racycline and ampicillin. Some of the isolates in this study were found to harbor multiple resistant genes conferring resistance to two or more different classes of antibiotics. The repertoire of antibiotic-resistant genes found in the E. coli pathotypes might serve as a pointer to the possible presence of other antidrug-resistant genes conferring re- sistance to other classes of antibiotics that were not targeted in this study, and our nding is in line with other reports on the detection of multiple antibiotic-resistance gene in some commensal and pathogenic strains of E. coli