Preparation of callus induction medium: The media including MS and B1 (Doley and Saunders, 1989) were applied. NAA, 2,4-D, IAA, TDZ and BAP from auxin and cytokinin types were used as the plant growth regulators. Three types of explants were cultured in 18 MS medium with different concentration of plant growth regulators and Gamborg’s vitamins was combined at all mediums (Table 1). Hormone-free MS (CIM0) was considered as control and B1 medium was prepared according to its formulation. Five explants cultured in each Petri dish containing 30 mL of medium were regarded as one treatment and each treatment was repeated three times. The primary results were obtained after three weeks of culture and the average of data were calculated.