The FTC method was used to measure the amount of peroxide at
the beginning of peroxidation. This assay was carried out as described
by Kikuzaki and Nakatani (1993). Samples of 4 mg each
were weighed and dissolved in 3.9 mL of distilled water. Subsequently,
4.1 mL of 2.52% linoleic acid in absolute ethanol, 8 mL of
0.05 M phosphate buffer (pH 7.0) and 3.9 mL of distilled water
were added and placed in an oven at 40 C in the dark. To a
0.1 mL aliquot of this solution, 9.7 mL of 75% ethanol and 0.1 mL
of 30% ammonium thiocyanate were added. Precisely 3 min after
the addition of 0.1 mL of 0.02 Mferrous chloride in 3.5% hydrochloric
acid to the reaction mixture, the absorbance of red colour was