Introduction Pullulan, one of the commercially important exopolysaccharides, is produced by Aureobasidium pullulans. The applications ofpullulan in pharmaceutical, food and biomedical fields are well rec-ognized [1–10]. In spite of its commercial value, pullulan appears to be economically less competitive owing to its high production cost [11].Cost of substrate contributes significantly to the pullulan production [11–13]. Previous studies have shown that the cost of production could be cut down by 30% if suitable cost-effectivemedium is employed in fermentation [11]. In order to reduce theraw material cost, production of pullulan by Aureobasidium pullulans has been investigated extensively using various lignocellulosic wastes, in submerged fermentation [10–27]. However, studies on pullulan production by solid-state fermentation are limited.Solid-state fermentation is superior to submerged fermenta-tion owing to its inherent advantages. It is simple, requires less energy and provides a better yield [26]. In our previous work,we have successfully produced pullulan from a low cost carbonsource, Asian Palmyra palm kernel, in solid state fermentation