Spawn Preparation
Selected good quality sorghum grains free
from pest and moulds. Boil the grains
submerged in clean water for 20 30
minutes. When the grains become soft, it
was removed and was spread evenly on a
cotton cloth to drain out the water and to
cool the grains. Mixed 3 % chalk powder
(30g / kg of grain) for adjusting the pH and
to keep the grains loose. Filled 250 gms of
grain in cleaned and dried glucose bottle of
500ml capacity polypropylene bags and
plugged the mouth of the bottle tightly with
non absorbent cotton. Sterilize the bottles
in an autoclave by exposing to 121 c and
15lbs pressure / sq inch for 20 minutes.
After cooling transfered the bottles to
inoculation chamber.