Effect of moisture on cell disruption and sugar extraction
Fig. 1 compared as well, the effect of the dryness on the microalgal
biomass disruption/sugar extraction with the methods evaluated
for wet biomass.
The results depicted in this figure showed a better sugar extraction
from the dried biomass, mainly with the acids as a pre-treatment
method (both for sulfuric and chloridric acids). The sugar
release using dried biomass increased 55% when compared to the
results obtained with the wet biomass. The acid (HCl and H2SO4)
pre-treatment method revealed to be the best for disruption/sugar
extraction for both dried and wet biomass.
From this figure it can also be concluded that drying the biomass
has a synergistic effect on the sugar extraction at 120 _C, as
well as with homogenization. With the alkaline pre-treatment
and with the other physical methods excluding temperature, the
biomass drying level seems to be irrelevant. The disruption method
using NaOH (3 N) at 120 _C confirmed a low efficiency even using
dried biomass. The obtained results after drying did not reach
the ones using only the temperature (120 _C, in autoclave), and
are in agreement with the hypothesis suggested above concerning
sugar degradation due to the strong alkaline treatment, and are in
accordance with the Hendriks and Zeeman (2009) work.
With the sonication and the bead-beading disruption methods,
drying also did not improve the disruption of the cell wall nor the
sugar extraction. The homogenization method was the worst physical
method for wet biomass (1.6 ฑ 0.02% g eqglu g dw_1), however,
it presented the highest efficiency for the dried one
(3.1 ฑ 0.2% g eqglu g dw_1).
Effect of moisture on cell disruption and sugar extractionFig. 1 compared as well, the effect of the dryness on the microalgalbiomass disruption/sugar extraction with the methods evaluatedfor wet biomass.The results depicted in this figure showed a better sugar extractionfrom the dried biomass, mainly with the acids as a pre-treatmentmethod (both for sulfuric and chloridric acids). The sugarrelease using dried biomass increased 55% when compared to theresults obtained with the wet biomass. The acid (HCl and H2SO4)pre-treatment method revealed to be the best for disruption/sugarextraction for both dried and wet biomass.From this figure it can also be concluded that drying the biomasshas a synergistic effect on the sugar extraction at 120 _C, aswell as with homogenization. With the alkaline pre-treatmentand with the other physical methods excluding temperature, thebiomass drying level seems to be irrelevant. The disruption methodusing NaOH (3 N) at 120 _C confirmed a low efficiency even usingdried biomass. The obtained results after drying did not reachthe ones using only the temperature (120 _C, in autoclave), andare in agreement with the hypothesis suggested above concerningsugar degradation due to the strong alkaline treatment, and are inaccordance with the Hendriks and Zeeman (2009) work.With the sonication and the bead-beading disruption methods,drying also did not improve the disruption of the cell wall nor thesugar extraction. The homogenization method was the worst physicalmethod for wet biomass (1.6 ฑ 0.02% g eqglu g dw_1), however,it presented the highest efficiency for the dried one (3.1 ฑ 0.2% g eqglu g dw_1).
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