The formulations were administered intraperitoneally to the rats 30 min after hyperuricemia induction.
For normal and hyperuricemic controls, the animals were treated only with the vehicle. Then, the animals were placed in metabolic cages with 100 mL of tap water. The urine collected in graduated
tubes and the water intake were measured for 5 h after the
treatments. Finally, the animals were anesthetized with an association of ketamine and xylasine (40 and 87 mg/kg, respectively),
administered intraperitoneally, in order to collect the blood from
abdominal aorta. Blood samples were maintained at room temperature until blood coagulation and, afterwards, the serum was
obtained after centrifugation at 3000gfor 10 min. The serum and
urine samples were stored at20°C until uric acid quantification,
which was performed by colorimetric method, using a standard
diagnostic kit (Bioclin, Brazil), according to manufacturer’s
instructions