Cowpea plants at the first fully expanded trifoliate leaf stage
were subjected to three treatments. (1) 200 m
NaCl prepared
in the nutrient solution daily, applied during six consecutive
days (salt treatment). (2) 3 d 200 m
NaCl treatment, followed
by intensive pot washings with tap water until the electric
conductivity of percolate reached the same level of control,
and subsequent irrigation with nutrient solution up to 70%
of pot capacity, similar to control plants (recovery treatment).
(3) Watering with nutrient solution up to 70% of pot capacity
during 6 d (control). Control and salt-treated plants were
harvested daily during six consecutive days; those under the
recovery treatment were harvested at 1, 2 and 3 d after NaCl
withdrawal. Before each harvesting (at 08:00 h), plants were
transferred to a controlled growth room (27
°
C, 72% RH,
230 μmol m
−
2
s
−
1
photon flux density) for 2 h. Next the leaves
(mature trifoliate leaf ) were sampled, frozen in liquid nitrogen
and immediately utilized for biochemical determinations.
Cowpea plants at the first fully expanded trifoliate leaf stage
were subjected to three treatments. (1) 200 m
NaCl prepared
in the nutrient solution daily, applied during six consecutive
days (salt treatment). (2) 3 d 200 m
NaCl treatment, followed
by intensive pot washings with tap water until the electric
conductivity of percolate reached the same level of control,
and subsequent irrigation with nutrient solution up to 70%
of pot capacity, similar to control plants (recovery treatment).
(3) Watering with nutrient solution up to 70% of pot capacity
during 6 d (control). Control and salt-treated plants were
harvested daily during six consecutive days; those under the
recovery treatment were harvested at 1, 2 and 3 d after NaCl
withdrawal. Before each harvesting (at 08:00 h), plants were
transferred to a controlled growth room (27
°
C, 72% RH,
230 μmol m
−
2
s
−
1
photon flux density) for 2 h. Next the leaves
(mature trifoliate leaf ) were sampled, frozen in liquid nitrogen
and immediately utilized for biochemical determinations.
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