Aseptic Technique is a good method to prevent undesirable microorganism and contamination during transferring or handling the microorganism. Streak plate technique is used for isolation of single colonies from different type of microorganism on the same media. Moreover, streak plate technique should be required more attention on streaking from one quadrant to another quadrant otherwise the colony of microorganisms will not be isolated. Furthermore, Gram staining is used to divide two different groups of bacterial including Gram positive bacteria and Gram negative bacteria depend on structure and chemical composition of their cell wall.