was grown overnight at 25 °C in 20 mL YPD broth (1% yeast extract, 2% bacteriological peptone, 2% dextrose; Sigma-Aldrich, St. Louis, MO, USA). Cells were recovered by centrifugation, washed, resuspended in sterile water and counted with a Thoma haemocytometer to obtain a 1 x 109 CFU/mL concentration in 25 mL of water containing 2% sodium alginate.