1. The small, free-living nematodes, Turbatrix aceti, Rhabditis anomala and Panagrellus redivivus, can be readily grown in axenic culture on a scale suitable for biochemical studies.
2.
2. A number of observations related to the growth requirements of the organisms are reported.
3. An improved yield of Caenorhabditis briggsae has been attained by addition of autoclaved casein to the medium, permitting a reduction in the amount of liver extract required. This procedure also appears to be effective for T. aceti