Glucose was subjected to ethanol fermentation by yeast stain for both of non-immobilized and
immobilized yeast under anaerobic condition (pH 4.8; 32oC; mixing rate: 150 rpm). To the fermentation
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medium, 2.5% of yeast strain was added. The fermentation was performed in 250 ml flasks which
contained 100 ml of medium in scientific orbital shaker for 72 hours.