All the treatments were incubated in an incubator shaker at 150 rpm for 8 weeks at 40°C. The experimental design for the three Pseudomonas spp. comprised of two treatments each i.e. T1 (Pseudomonas + natural polyethylene) and T2 (Pseudomonas + synthetic polyethylene) with controls i.e. C1 (natural polyethylene) and C2 (synthetic polyethylene) and blank (Pseudomonas). A blank for every
Pseudomonas spp. was used to examine the viability of the bacteria and check for any contamination.