Later, the saccharification and ethanol fermentation of the JA juice
from previous step were performed simultaneously in batch mode at
30 °C in partially anaerobic conditions, in the same Erlenmeyer flask
of 500 mL. Inoculation was done with 3 g dry wt of the yeast described
above per litre of juice. Yeast had been previously activated in a liquid
medium (13 mL distilled water and 0.5 g sucrose per gram dry wt.) in
a shake flask at 40 °C for 20 min.