The calli derived from anthers
were transferred to differentiation medium to induce
adventitious shoot formation. In order to select the optimum
medium for shoot production, MS, MH (MS salts
and microelements of H medium [1]), and WPM [7]
media were tested. At last, the shoots about 1 cm in
length were detached and transferred to rooting
medium containing 0.2 mg/l NAA. After 20–25 days,
the rooted plantlets were transplanted in soil. In present
study, all the tissue cultures were maintained at
25°ëunder the 16 h light/8 h dark cycle. For callus induction
from anthers, medium contained 30 g/l sucrose, and
media for other cultures contained 20 g/l sucrose. All
the media were solidified with 0.8% agar. In this study,
all the hormone reagents and antibiotics were purchased
from Duchefa (Haarlem, The Netherlands).