2.2. Preparation of germinated brown rice (GBR)
Malaysian local brown rice (Oryza sativa L., variety MR220) was
obtained from a major rice miller (BERNAS) in Selangor, Malaysia.
Brown rice was sterilised in 0.1% sodium hypochlorite for 30 min
and then washed in sufficient water. Germination of brown rice
was carried out as previously described by Suzuki and Maekawa
(2000), with a slight modification. Four kilograms of brown rice
was soaked in 120 L of water at a controlled temperature of
30 C. The soaking water was changed every 24 h. The WR, BR
and GBR were then ground into powder and kept in airtight containers
at 20 C before being analysed.