The
MTC was determined using a bacterial concentration of 103 CFU/ml.
The MTC investigated was undertaken in order to characterize the
level of HE that did not affect the growth of the target bacteria. Since
the ultimate goal of this experiment is to combinemultiple antimicrobial
compounds in a lower concentration the MTC investigation was important
to our research. The MIC and MTC values were determined
based on the average MIC or MTC of 3 separate growth kinetic and
were expressed in part per million (ppm or mg/l). As a control, a solution
of Tween (10%; w/w) was assayed and it did not show any growth
inhibition against target bacteria. In this study, the results obtained for
the PC were used to compare our results with other experiments published
in the literature. Each experiment was done in triplicate.