temperature. In addition, the effect of particle
size of sugarcane bagasse in hydrolysis
was studied. Then, the butanol fermentation
utilizing the sugarcane bagasse hydrolysates
as substrate without removing inhibitors was
preliminary investigated. The microorganisms
used were Clostridium butyricum (TISTR 1032),
Clostridium sporogenes (TISTR 1452), Clostridium
beijerinckii (TISTR 1461) and Clostridium
acetobutylicum (TISTR 1462).