Some PCR-based approaches, however, have employed PCR
primers containing microsatellite motifs at the 34 end and three or
more non-repetitive bases at the 54 end (9,10,12). The 54 bases are
expected to ‘anchor’ the primer to the 54 end of a microsatellite.
In practice this approach has proven difficult to optimise for
complex genomes and there has been little evidence that variation
in microsatellite repeat length at the primer binding sites has been
preserved (9). These difficulties arise because the PCR primers