MATERIALS AND METHODS
Soil sampling and analysis
Four soil samples were collected from a citrus garden located in the city of Darab (Iran) with the following characteristics: soil 1, It was polluted by glyphosate (GP) at a slow rate (in 12 years time) with agricultural activities; Soil 2, the GP was added to soil 1, one month before sampling; soil 3, GP was added to soil 1 in the day of sampling; soil 4, non polluted, as a control.
All the soil samples were taken from the surface layer to a depth of 10 cm and moved to the laboratory in the sterilized containers.
Air-dried sub-samples were sieved (< 2 mm) for physical and chemical analyses (Table 1).