The present study was conducted to describe the
post-thaw quality of semen collected from mature
swamp buffalo sires for freezing-thawing and ultimate
use of AI in Thailand, in relation to the season and year
when the semen was primarily processed. Sperm
plasma PMI was assessed using SYBR-14/PI, while
PMS was measured using Annexin-V/PI. Both sperm
attributes, which are of utmost relevance for the
fertilizing ability of a spermatozoon, appeared significantly
(P < 0.001) higher (as proportions of
spermatozoa per frozen-thawed sample) when the
semen was collected/processed in winter (55–56%),
and were lowest during the rainy season (40–43%). The
proportion of linearly motile spermatozoa, which is the
parameter that seems of higher relevance for fertilization
in vitro and among the attributes that link to in vivo
fertility [50], was measured by CASA. Linear Mot was
around 50% at (T0) and 35% at (T60), without
significant differences between the seasons ascribed
to the location of the bull sires and the production unit in
tropical Thailand. The average of VSL, VAP, and VCL
were significantly (P < 0.05–0.001) higher in the rainy
season than in winter or summer, while ALH was
highest (P < 0.05) in summer (P < 0.05). All kinematic
variables except ALH significantly decreased following
incubation at 38 8C for 60 min (T60).
The present study was conducted to describe thepost-thaw quality of semen collected from matureswamp buffalo sires for freezing-thawing and ultimateuse of AI in Thailand, in relation to the season and yearwhen the semen was primarily processed. Spermplasma PMI was assessed using SYBR-14/PI, whilePMS was measured using Annexin-V/PI. Both spermattributes, which are of utmost relevance for thefertilizing ability of a spermatozoon, appeared significantly(P < 0.001) higher (as proportions ofspermatozoa per frozen-thawed sample) when thesemen was collected/processed in winter (55–56%),and were lowest during the rainy season (40–43%). Theproportion of linearly motile spermatozoa, which is theparameter that seems of higher relevance for fertilizationin vitro and among the attributes that link to in vivofertility [50], was measured by CASA. Linear Mot wasaround 50% at (T0) and 35% at (T60), withoutsignificant differences between the seasons ascribedto the location of the bull sires and the production unit intropical Thailand. The average of VSL, VAP, and VCLwere significantly (P < 0.05–0.001) higher in the rainyseason than in winter or summer, while ALH washighest (P < 0.05) in summer (P < 0.05). All kinematicvariables except ALH significantly decreased followingincubation at 38 8C for 60 min (T60).
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