The objective of this study was to evaluate the resistance of surface-attached Listeria innocua and Listeria monocytogenes to sanitizing agents under laboratory conditions. Six strains of L. monocytogenes and one strain of L. innocua were attached to stainless steel or aluminum coupons that had been cut from a used deli meat slicer. One cleaner/sanitizer and two sanitizers were tested against the attached cells. No sanitizer caused more than a 1.5 log CFU/cm2 reduction of Listeria when treated and untreated coupons were compared. Many delicatessens are using sanitizing wipes during operating times. Therefore, the best performing sanitizer, sanitizer C, from the first experiment was applied with a variety of cleaning cloths and compared with a commercial sanitation wipe. No cloth produced more than a one log reduction compared to controls.