studied the effect of ultrasound irradiation temperature on the size distribution of ice crystals in agar gel samples. The results indicated that the size distribution of ice crystals was affected by the temperature of ultrasound induced nucleation. Decrease of the nucleation temperature resulted into smaller ice crystals . Kiani et al. also studied that the effect of different ultrasound parameters (intensity, duration and duty cycle) on the size distribution of ice crystals in agar gel samples. They found that the size of ice crystals was smaller in ultrasound irradiated samples compared to that in unirradiated samples. However, no quantifiable relationship between these evaluated parameters and the crystal size was observed. Saclier et al. assessed the effect of degree of supercooling and level of acoustic power on the size and shape of ice crystals in mannitol solution which was frozen using ultrasound-assisted freezing.