The
tissues were mixed well and then 10% SDS was added to make a final concentration of 0.1%. It was then
incubated at 650C for 1 hour with periodical gentle mixing during the incubation. Equal volume of
chloroform: isoamyl alcohol (24:1) was added, mixed well, and centrifuged at 2000 g for 15 min. The chloroform: isoamyl alcohol extraction was repeated until the interface became clean.