The inclusion of whole amaranth flour in the bread formulation significantly increased the amount of phytate from non-detectable values to 2.35 mmol/g (d.m.) for the control sample and 40 g/100 g WAF, respectively. The same tendency was observed in InsP5, and even more in InsP4 and InsP3, which increased significantly with the inclusion of amaranth flour. Phytates are mainly present in outer layers of the grain, and during the breadmaking process endogenous phytate-degrading enzymes with the potential to hydrolyse phytates to InsP5eInsP3 could be active (Sanz-Penella et al., 2008, 2009).