4. Conclusion
Generally saccharification step is carried out by commercially
available cellulase enzyme which is very expensive. This preliminary study revealed that ethanol production from sugar-cane
bagasse is possible by intact fungal organisms as a source of
cellulase and hemi-cellulase enzymes; through the solid state fermentation by T. viride F-94 or A. terreus F-98. This was followed
by the hydrolysate fermentation by C. tropicalis Y-26 or S. cerevisiae Y-39 through separate hydrolysis and fermentation process. the ethanol yield in this study was 75.36, 61.59 gallon/
ton, respectively. This ethanol yield was 1.48, 1.81 times lower
than the theoretical yield (111.5 gallon/ton as per National
Renewable Energy Laboratory (NREL) calculation, which is
found at (http://www1.eere.energy.gov/biomass/ethanolyieldcalculation.html). In this study, a significant removal of lignin
from bagasse was achieved through an eco-friendly process,
which resulted in high production of ethanol. Further study is
needed to optimize the conditions for maximum production of
ethanol from bagasse.