CONCLUSIONS
Most of the models used to assess the adhesion of
probiotics in vitro represent simplifications of in vivo
conditions. However, some recommendations may be
made to bring the in vitro observations more in line with
the in vivo situation (Table II). Great care must be taken to
extrapolate from in vitro observations. In vitro models are
of great help in selecting probiotic candidates and are
indispensable instruments in the study of adhesion mechanisms.
Nonetheless, transient colonization and in vivo adhesion
of probiotics must be determined in vivo. Due to hostspecific
physiology, anatomy and intestinal microbiota
factors, this should be performed in the host for which
the probiotic is intended.
Considering all the factors influencing in vivo adhesion
that cannot be simulated in vitro, the validity of results from
in vitro adhesion assays remains extremely uncertain. The
assumed correlation between in vitro and in vivo adhesion
therefore requires urgent investigation, using adherent and
low-adherent isogenic strains with in vitro and in vivo
CONCLUSIONSMost of the models used to assess the adhesion ofprobiotics in vitro represent simplifications of in vivoconditions. However, some recommendations may bemade to bring the in vitro observations more in line withthe in vivo situation (Table II). Great care must be taken toextrapolate from in vitro observations. In vitro models areof great help in selecting probiotic candidates and areindispensable instruments in the study of adhesion mechanisms.Nonetheless, transient colonization and in vivo adhesionof probiotics must be determined in vivo. Due to hostspecificphysiology, anatomy and intestinal microbiotafactors, this should be performed in the host for whichthe probiotic is intended.Considering all the factors influencing in vivo adhesionthat cannot be simulated in vitro, the validity of results fromin vitro adhesion assays remains extremely uncertain. Theassumed correlation between in vitro and in vivo adhesiontherefore requires urgent investigation, using adherent andlow-adherent isogenic strains with in vitro and in vivo
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