Morris water maze
Morris water maze tests were conducted as described previously (Baglietto-Vargas et al., 2013; Billings et al., 2005; Medeiros et al., 2011). Mice were trained to swim to a 14 cm diameter circular Plexiglas platform submerged 1.5 cm beneath the surface of the water and invis- ible to the mice while swimming. The platform was located in a fixed position, equidistant from the center and the wall of the tank. Mice did four training trials per day. During each trial, mice were placed into the tank at one of four designated start points in a pseudorandom order. Mice were trained for as many days as needed to reach the train- ing criterion of 25 s (escape latency). If the mice failed to find the plat- form within 60 s, they were manually guided to the platform and allowed to remain there for 5 s. The probe was assessed 24 h after the last training session and consisted of a 60-s trial without the platform. Performance was monitored with the EthoVision XT video tracking system.