High-pressure treatments
Samples of spores and vegetative cells were pressurized using a 2.2-L isostatic pressure vessel (Model 4-04486–1, Engineered
Pressure Systems, Inc., Haverhill, Mass. U.S.A.). The pressuretransmitting fluid was water containing 5% (v/v) Hydrolubric 120-B
(Houghton Intl. Inc., Valley Forge, Pa. U.S.A.).