Aragonite statoliths dissected from paralarvae reared in treatment pCO2 had 25% lower surface area (F1,55 = 70.722, p<0.001; Fig. 4). There was no significant difference between trials (F1,54 = 2.175, p<0.146). While statoliths from the control CO2 treatment were normally shaped, statoliths from high CO2 paralarvae typically exhibited abnormal and irregular shapes and were more porous (Fig. 5). In addition, SEM images of statoliths from the control treatment showed crystal size (submicron), shape (acicular), orientation (aligned along long-axes) and arrangement of crystal bundles (arrays radiating out from the primordium) that were typical of statoliths formed under ambient pCO2 conditions and consistent with paralarval statoliths of other squid species [48]. This highly organized ultra-structure was not observed in statoliths accreted under elevated pCO2. The distribution of grades given to statoliths varied significantly between treatments (χ2 = 35.796, df = 2, p<0.001; Fig. 6).
การแปล กรุณารอสักครู่..
