75.3 g of chitosan flakes was dissolve in 1 l of 3.0% (v/v) acetic acid solution. The chitosan solution obtained was filtered through a polystyrene sieve with mush 100 μm to remove impurities. The filtered chitosan solution was pumped with a peristaltic pump through a glass pipette. Upon contact with 1 M sodium hydroxide solution, the anti-solvent induces the formation of chitosan gel beads. The wet chitosan beads was rinsed several times with distilled water and filtered to remove any residual sodium hydroxide.