2.3. Hyphal phase product inoculation
In all experiments reporting hyphal phase growth,
commercially available yogurt was purchased from a retailer (Geneva, NY) in
850.5 g (32 oz) units and 30 g of yogurt was transferred to individual
stomacher bags. Yogurt was inoculated with M. circinelloides (approximately
104 CFU/g yogurt), homogenized, and transferred by weight
into sterile stomacher bags. Yogurt samples were stored aerobically at
designated storage temperatures (4 °C, 15 °C, and 25 °C) for the growth
study. All data comparing growth rates of various M. circinelloides isolates
(Fig. 1) were collected from yogurt incubated at 25 °C.