The ORAC assay was done
following the analytical procedures of Huang, Ou, Hampsch-Woodill,
Flanagan, and Prior (2002). Briefly, 150 μl fluorescein (20 nM) was
added to a 96-well black plate, followed by 25 μl of each of the following
solutions: blank (sodium phosphate buffer), Trolox standard and diluted
sample extract (50 μl sample extracts were added to 10 ml sodium
phosphate buffer).