One hundred mg Micrococcus lysodeikticus was suspended in 500 ml of PBS (0.04 mol L-1 phosphate buffer solution,pH 6.4) 42 มิวแอล serum sample was added to 5 mL of Micrococcus lysodeikticus suspension.
The adsorbance at 540 nm. Were recorded after 30 s (Ao) and 4 min 30 s (A1) respectively. Lysozyme activity was calculated as follows: