An agar diffusion method to select the active isolates against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 was
used. Furthermore, a combination approach consisting thin layer chromatography-bioautography (TLC-bioautography) analysis and restriction
fragment length polymorphism (RFLP) analysis using HaeIII (BsuRI) restriction enzyme digestion were applied