the contents of ALA, EPA and DHA in beef, and
decreased the n-6/n-3 PUFAs ratio by 82.2 % compared
to the transgenic negative calf.
Our results provide evidence that new breed of
cattle containing high level of n-3 PUFAs can be
created in a relatively short time by using transgenic
technology. However, the high neonatal mortality of
SCNT animals are still problems that hindering the
utilization of transgenic animals (Kang et al. 2001). In
the present study, septicemia and organ developmental
abnormalities were the major causes of neonatal
mortality (Table 2), consistent with the previous
studies (Brisville et al. 2013). These abnormalities
may partly result from the incomplete reprogramming
of somatic cells or abnormal epigenetic modification