The calculated
amount of fiber fraction, SAA pretreated fiber fraction or cellulose along with 10 mL of YP medium, 10 mL of
0.05 M citrate buffer [25], 10 mL of S. cerevisiae inoculum,
enzyme Experimental Optimase CX 15L (190 FPU/gglucan),
and water were added to the Erlenmeyer flask to
bring the total slurry weight to 200 g.