2.2.3. Ultrasound assisted extraction procedure and apparatus
2.2.3.1. Ultrasound bath
Ground rosemary leaves were immersed into the solvent and the mixture was introduced in an ultrasonic bath (Prolabo, Labover, France) during 30 min. Temperature of water bath was maintained at 40 °C and checked during extraction with an external temperature probe. Each experiment was performed in triplicate.