In the last years, with the understanding increase of the disinfection and sterilization process in hospitals, the
importance of the cleaning step in the whole process efficacy has been raised [1] [2].
The presence of residual tissue and other body fluids on the instruments can result in layers of organic material
that may be difficult to remove and disinfect, and this situation is aggravated by the formation of biofilms [3].
Biofilms are composed by a population of microorganisms in an extra cellular matrix adhering to surfaces in
which sufficient moisture is available. Bacteria growing as surface associated biofilms are much harder to treat
with antimicrobials, potentially leading to dangerous infections [4]. Based on that, the importance of biofilm re