Anthocyanins from purple carrots were extracted by using the
method of Fuleki and Francis (1968) modified by Colin and
Peter (1980).Twenty gm of purple carrots was mixed with
100 ml ethanol acidified with 0.01% citric acid and macerated
in a warring blender at full speed for 5 min. The mixture was filtered
by Whatman No. 1 filter paper through a Buchner funnel.
The residue on the filter paper was washed rapidly with the
extracting solvent until collecting of about 450 ml from extracted
pigment carrot. The extract was concentrated in a rotary vacuum
evaporator at >40 C until the solvent evaporated.