The different steps of the data analysis are outlined in the sheet ”Analysis protocol - All tools” in the analysis template (Additional file 1) and in Table 3. Figure 1 shows the order and possible combinations of the analysis steps. Rather than having one procedure performing all steps the user must run the macros for the different steps. This allows for flexibility regarding what steps to include in the analysis. Between the steps the user also has the option to change the data that is going to be used, by copying the relevant data to the sheet “InData”. This allows for flexibility regarding what data to use in each step of the analysis. The corresponding procedures and input and output data sheets for the different analysis steps are shown in Table 3.