1.0 g freeze-dried samples was dissolved in 99 ml mobile
phase (50 mM phosphate buffer containing 0.15 M NaCl,
pH ¼ 7.2). The sample was loaded onto GE Superdex Peptide
10/300 GL column (i.d. 10 300 nm), eluted with mobile phase
at a flow rate of 0.5 ml/min and monitored at 220 nm. A molecular
weight calibration curve was obtained from the following
standards: Cytochrome C (12,500 Da), Trasylol (6512 Da),
Glutathione Disulfide (615 Da), Reduced Glutathione (310 Da)
and Glycine (75 D