In addition to the biologically nutritious components present in wine, there can be substances that are
harmful to human health. For this reason, the purpose of this study was to evaluate the contents of
volatile compounds and food additives in blackberry wine samples produced from conventionally and
organically grown blackberries and compare their concentrations with the maximum acceptable levels in
wines for the first time. Volatile compounds were analyzed in 15 blackberry wines using a GC-FID
method, while the contents of food additives were determined using a newly developed HPLC
method. Both methods were validated for their linearity, sensitivity, repeatability, and intermediate
precision. The major volatile compound in blackberry wine samples was ethanol (7.11e15.33%). The
second most prevalent volatile compound found in all blackberry wine samples was methanol (174.2
e641.4 mg/L), which is highly toxic. The amount of ethyl acetate in the investigated samples ranged from
53.8 to 188.4 mg/L, while propan-1-ol was found in three of the organic samples. Although isoamyl
alcohol was found in all samples, the measured values ranged from 56.7 to 226.9 mg/L. The obtained
results demonstrated that the concentrations of benzoic acid in the analyzed blackberry wines varied
from 4.36 to 18.55 mg/L. The presence of sorbic acid has was detected in seven samples, and the values
were in the range of 1.43e54.48 mg/L. Salicylic acid is a natural component of blackberry fruit, and the
contents of this phytochemical in the studied wines were between 1.99 and 13.55 mg/L. The saccharin
concentrations were detected in three samples, and the highest value was 31.51 mg/L. The concentrations
of amyl alcohol, acesulfame, aspartame and caffeine were found to be below the quantitation limits of
the method in the investigated blackberry wines. Furthermore, the obtained data provide evidence that
the contents of methanol in blackberry wines were above the permissible limits, except for four conventional
blackberry wines. Thus, the concentrations of analyzed volatile substances measured in
blackberry wines necessitate more extended investigations of these volatile compounds in this popular
fruit wine.
In addition to the biologically nutritious components present in wine, there can be substances that areharmful to human health. For this reason, the purpose of this study was to evaluate the contents ofvolatile compounds and food additives in blackberry wine samples produced from conventionally andorganically grown blackberries and compare their concentrations with the maximum acceptable levels inwines for the first time. Volatile compounds were analyzed in 15 blackberry wines using a GC-FIDmethod, while the contents of food additives were determined using a newly developed HPLCmethod. Both methods were validated for their linearity, sensitivity, repeatability, and intermediateprecision. The major volatile compound in blackberry wine samples was ethanol (7.11e15.33%). Thesecond most prevalent volatile compound found in all blackberry wine samples was methanol (174.2e641.4 mg/L), which is highly toxic. The amount of ethyl acetate in the investigated samples ranged from53.8 to 188.4 mg/L, while propan-1-ol was found in three of the organic samples. Although isoamylalcohol was found in all samples, the measured values ranged from 56.7 to 226.9 mg/L. The obtainedresults demonstrated that the concentrations of benzoic acid in the analyzed blackberry wines variedfrom 4.36 to 18.55 mg/L. The presence of sorbic acid has was detected in seven samples, and the valueswere in the range of 1.43e54.48 mg/L. Salicylic acid is a natural component of blackberry fruit, and thecontents of this phytochemical in the studied wines were between 1.99 and 13.55 mg/L. The saccharinconcentrations were detected in three samples, and the highest value was 31.51 mg/L. The concentrationsof amyl alcohol, acesulfame, aspartame and caffeine were found to be below the quantitation limits ofthe method in the investigated blackberry wines. Furthermore, the obtained data provide evidence thatthe contents of methanol in blackberry wines were above the permissible limits, except for four conventionalblackberry wines. Thus, the concentrations of analyzed volatile substances measured inblackberry wines necessitate more extended investigations of these volatile compounds in this popularfruit wine.
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